Plasmid

Part:BBa_K530038

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)

pRSBB425 LEU2 2Micron Episomal Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and LEU2 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1116
    Illegal XbaI site found at 6309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1116
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1116
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1116
    Illegal XbaI site found at 6309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1116
    Illegal XbaI site found at 6309
    Illegal NgoMIV site found at 2799
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4479
    Illegal SapI site found at 3396


Plasmid Map

PRS425.png

[edit]
Categories
Parameters
None